Journal: iScience

Article Title: Gata2 heterozygous mutant mice exhibit reduced inflammatory responses and impaired bacterial clearance

doi: 10.1016/j.isci.2021.102836

Figure Lengend Snippet: Reduced cytokine levels in G2 Het mice (A) Experimental workflow of multiplex cytokine immune analysis by MAGPIX (n = 4 for the PBS-treated group and n = 5 for the LPS-treated group in WT and G2 Het ). (B) Heatmaps visualizing the fold changes in mean cytokine concentration in the G2 Het mice over the WT mice in peritoneal cavity lavage fluid (PEC) and plasma upon LPS stimulation. Note that almost all cytokines (30/32) in PEC were decreased in the G2 Het mice compared with the WT mice (left column). IFN-γ, IL-12p40, and IL-17A were most diminished in the plasma of G2 Het mice (∗ in right column). (C and D) Quantification results show that IFN-γ, IL-12p40, and IL-17A levels upon LPS stimulation were diminished in peritoneal lavage (C) and plasma (D). Basal levels of IL-12p40 and IL-17A in the plasma of vehicle (PBS)-treated G2 Het mice were also decreased (insets in D). Results were calculated from two experiments. Data in C and 2D are represented as the mean ± SEM. Dots in C and 2D represent independent biological replicates. p Values were calculated with unpaired t tests for C and 2D. LOD, limit of detection; n.s., not significant. See also Figure S3 for further information.

Article Snippet: Multiplex cytokine analysis was performed with diluted samples and a 6-plex/32-plex Mouse Cytokine/Chemokine Magnetic Bead Panel (Merck Millipore, Barrington, MA) on a MAGPIX (Merck Millipore, Barrington, MA) according to the manufacturer’s instructions.

Techniques: Multiplex Assay, Concentration Assay, Clinical Proteomics